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Conditions of optimal recombinant Mouse GFRAL/GFR alpha-like Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005603: Mouse GFRAL/GFR alpha-like Protein with the N-His-Avi Tag
Source: The recombinant Mouse GFRAL/GFR alpha-like Protein, (amino acids Gln20-Glu350) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse GFRAL/GFR alpha-like protein with the His-Avi tag at the N-terminus has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse GFRAL protein with the N-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse GDF15 protein with the N-hFc tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse GFRAL/GFR alpha-like Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Mouse GFRAL/GFR alpha-like Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following GFRAL Protein :
Biotinylated Cynomolgus GFRAL Protein
Biotinylated Human GFRAL Protein
Biotinylated Mouse GFRAL Protein
Cynomolgus GFRAL Protein
Human GFRAL Protein
Mouse GFRAL Protein
Rat GFRAL Protein
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